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4547 The effect of metformin is also supported by a study done by Shaw et al on mice given a high-fat diet, where deletion of gene LKB1 (Liver Kinase B1), which is a crucial enzyme phosphorylating the catalytic AMPK domain led to the deactivation of AMPK leading to elevated glucose levels, hence nullifying the glucose reducing metformin effect

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It is studied in controlled laboratory environments for biochemical pathway investigations and cellular process research

Dhitavat, S., Ortiz, D., Shea, T.B